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The engineered virus is replication incompetent and has a self-inactivating viral promoter (deleted U3). To summarize, the third generation lentiviral vectors employed in our studies are typically pseudotyped with a VSV-G envelope that allows stability and tropism for cells of multiple species including mice. We have previously published a detailed description of the lentiviral backbone and technique for packaging high titer virus for in vivo studies, and several of these protocols may be downloaded here by clicking on protocols. Kotton worked with the original pHAGE backbone during his postdoctoral fellowship in the Mulligan laboratory and the backbone has since been adapted to express the transgenes indicated on this page. The lentiviral system we employ is based on an HIV-1-based backbone, named ‘pHAGE’ (standing for plasmid HIV-1 Alex Gustavo George Enhanced) originally developed in the laboratory of Dr. PHAGE-EF1aL-TagBFP-W W=WPRE sequence L=long version of promoter Background on pHAGE: A third generation lentiviral vector These and other vectors are also available to Boston University Investigators at our CReM vector core webpage, please visit here for a more complete inventory.įor information on our iPS cell bank, please visit our CReM iPS Cell Core website. Kotton, serves on the Addgene Board of Directors. Some of our vectors are also available from Addgene, where our PI, Dr. pdf files by clicking on the relevant link below for each vector.
VECTOR NTI EXPRESS FOR FREE
Vector maps and sequences are available for free download as genbank formatted. Please visit our PROTOCOLS page for all protocols, including ESC/iPSC lung differentiation, lentiviral packaging and titering, or intratracheal lentiviral transduction of lung macrophages! Or take our annual hands-on 1 week course! Sign up through the CReM iPSC Core webpage. Requesting our cells is easy, click here for details!ĭifferentiating iPSCs in your own lab is a little harder, but we’ll show you how:
VECTOR NTI EXPRESS WINDOWS
Corrected an issue with clipped content at the top of the Keyboard Shortcuts and Gestures dialogs on Windows and Linux.Vectors, Protocols, and Cells So many cells, so little time…. VECTOR NTI EXPRESS TRIAL
Corrected an issue that prevented detecting a converted or extended trial license. Fixed an issue where feature annotations could be lost when adding a restriction site via silent mutation. Fixed an issue where an agarose gel fragment list could be clipped on Windows. Fixed an issue with printing genetic codes. Fixed importing a Vector NTI Express database with an atypical Java installation. Fixed a stability issue when switching between sequence trace and other window types on Windows or Linux. Fixed an issue with importing some PubMed references that include Unicode characters. Fixed a crash when trying to open a mirror to a mirror file in a Collection. Corrected an issue where collection folders were at times expanded automatically by mistake. Improved stability when mousing over stop characters (*) in protein alignments. Fixed an issue where some pull down controls were too narrow on Windows. Corrected messages in the New DNA/RNA file window that at time incorrectly indicate the topology of the sequence that will be created. Improve spacing of fragment controls in cloning windows. Fixed an issue where the application logo was clipped in the launch dialog on Linux. Improved stability when closing windows and quitting. Fixed an issue that prevented opening documents from Signals Notebook with non-Latin characters in their filename. (Reported by Tim Zhou and Anthony Picard). Fixed the keyboard shortcut for find protein sequence. Sped up loading sequences in agarose gels. Fixed an issue where "Copy Top Strand Bases" was not shown when viewing a Collection. VECTOR NTI EXPRESS ARCHIVE
Added a warning if the maximum file path on Windows would be exceeded in order to convert a multi-sequence FASTA archive with very long sequences names to a Collection. (Reported by Haijuan Yang, Justine Grisez, Viktoria Lytvyn, Rhiannon Carr and Edward Green). Fixed an issue that could result in duplicate primer binding sites or binding sites shown at the wrong location. Corrected an issue where selected feature types were not visible in the Manage Feature Types dialog on Windows. (Reported by Echo Pan, Justine Grisez and Clifford Dustin Rubenstein). Retain the order in which files were selected when aligning them to a reference sequence. Fixed an issue where some mirrored files on Windows were not shown in collections. Improved stability when showing context menus in Sequence view.
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